So you've swapped injectors, and the knock stays with the cylinder and doesn't follow the injector. Interesting. If the knock is related to the fuel injection system, it would be because either the injector is leaking (which alters the timing) or because the line is leaking (same thing). Since you say it idles rough, we know this to be a power stroke issue (no smart a$$ comments here, I'm talking about 1 of the 4 cycles
), and the fact that loosening the line stops the noise, indicates that combustion is taking place. Does the engine run smoother when you crack the nut? I wouldn't doubt it does. Since the problem only started when you changed the IP and injectors, it would seem foolish to suggest it is still anything but those components. However, I'm at a loss as to suggest anything that hasn't already been covered. I would swap the injector out completely, with one of your old ones (if you still have them) or obtain another and try it. If that doesn't work, replace the line. If you didn't screw up the firing order, and it's not leaking fuel, then it HAS to work!